Citrus phenylpropanoids and defence against pathogens. Part II: Gene expression and metabolite accumulation in the response of fruits to Penicillium digitatum infection
Identifieur interne : 001133 ( Main/Exploration ); précédent : 001132; suivant : 001134Citrus phenylpropanoids and defence against pathogens. Part II: Gene expression and metabolite accumulation in the response of fruits to Penicillium digitatum infection
Auteurs : Ana-Rosa Ballester [Espagne] ; M. Teresa Lafuente [Espagne] ; Luis Gonzalez-Candelas [Espagne]Source :
- Food chemistry [ 0308-8146 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Fruit.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid, Citrus (chemistry), Citrus (genetics), Citrus (immunology), Citrus (metabolism), Citrus sinensis, Flavones, Flavonoid, Fruit, Fruit (chemistry), Fruit (genetics), Fruit (immunology), Fruit (metabolism), Gene Expression, Gene expression, Infection, Metabolism, Metabolite, Orange, Penicillium, Penicillium (physiology), Phenols (chemistry), Phenols (immunology), Phenols (metabolism), Plant Diseases (genetics), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (immunology), Plant Proteins (metabolism).
- MESH :
- chemical , chemistry : Phenols.
- chemistry : Citrus, Fruit.
- genetics : Citrus, Fruit, Plant Diseases, Plant Proteins.
- immunology : Citrus, Fruit, Phenols, Plant Diseases, Plant Proteins.
- metabolism : Citrus, Fruit, Phenols, Plant Proteins.
- microbiology : Plant Diseases.
- physiology : Penicillium.
- Chromatography, High Pressure Liquid, Gene Expression.
Abstract
The effect of infection of Citrus sinensis (var. Navelina) fruits with Penicillium digitatum was studied at gene expression and metabolite levels. In this study, expression of genes involved in the phenylpropanoid pathway was studied in the flavedo (outer coloured part of the peel) and albedo (inner white part) in response to pathogen infection. Results of the time-course experiment showed that maximal expression of 10 out of 17 phenylpropanoid genes analysed occurred at 48 h post-inoculation, when decay symptoms started to appear, and mRNA levels either kept constant or decreased after 72 h post-inoculation. To further investigate the putative involvement of the phenylpropanoid pathway in the defence of citrus fruit, changes in the metabolic profile of both tissues infected with P. digitatum was studied by means of HPLC-PDA-FD. Metabolite accumulation levels along the time course suggest that flavanones, flavones, polymethoxylated flavones and scoparone are induced in citrus fruit in response to P. digitatum infection, although with different trends depending on the tissue.
Affiliations:
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Le document en format XML
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Teresa Lafuente</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7</s1><s2>Paterna, 46980-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Gonzalez Candelas, Luis" sort="Gonzalez Candelas, Luis" uniqKey="Gonzalez Candelas L" first="Luis" last="Gonzalez-Candelas">Luis Gonzalez-Candelas</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. 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Part II: Gene expression and metabolite accumulation in the response of fruits to Penicillium digitatum infection</title><author><name sortKey="Ballester, Ana Rosa" sort="Ballester, Ana Rosa" uniqKey="Ballester A" first="Ana-Rosa" last="Ballester">Ana-Rosa Ballester</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7</s1><s2>Paterna, 46980-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Lafuente, M Teresa" sort="Lafuente, M Teresa" uniqKey="Lafuente M" first="M. Teresa" last="Lafuente">M. Teresa Lafuente</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7</s1><s2>Paterna, 46980-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Gonzalez Candelas, Luis" sort="Gonzalez Candelas, Luis" uniqKey="Gonzalez Candelas L" first="Luis" last="Gonzalez-Candelas">Luis Gonzalez-Candelas</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Agroquímica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Av. Agustín Escardino 7</s1><s2>Paterna, 46980-Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Food chemistry</title><title level="j" type="abbreviated">Food chem.</title><idno type="ISSN">0308-8146</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Food chemistry</title><title level="j" type="abbreviated">Food chem.</title><idno type="ISSN">0308-8146</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Citrus (chemistry)</term><term>Citrus (genetics)</term><term>Citrus (immunology)</term><term>Citrus (metabolism)</term><term>Citrus sinensis</term><term>Flavones</term><term>Flavonoid</term><term>Fruit</term><term>Fruit (chemistry)</term><term>Fruit (genetics)</term><term>Fruit (immunology)</term><term>Fruit (metabolism)</term><term>Gene Expression</term><term>Gene expression</term><term>Infection</term><term>Metabolism</term><term>Metabolite</term><term>Orange</term><term>Penicillium</term><term>Penicillium (physiology)</term><term>Phenols (chemistry)</term><term>Phenols (immunology)</term><term>Phenols (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (immunology)</term><term>Plant Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Phenols</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Fruit</term><term>Plant Diseases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus</term><term>Fruit</term><term>Phenols</term><term>Plant Diseases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term><term>Fruit</term><term>Phenols</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Penicillium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Gene Expression</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Citrus sinensis</term><term>Expression génique</term><term>Métabolite</term><term>Fruit</term><term>Penicillium</term><term>Infection</term><term>Métabolisme</term><term>Orange</term><term>Flavone</term><term>Flavonoïde</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of infection of Citrus sinensis (var. Navelina) fruits with Penicillium digitatum was studied at gene expression and metabolite levels. In this study, expression of genes involved in the phenylpropanoid pathway was studied in the flavedo (outer coloured part of the peel) and albedo (inner white part) in response to pathogen infection. Results of the time-course experiment showed that maximal expression of 10 out of 17 phenylpropanoid genes analysed occurred at 48 h post-inoculation, when decay symptoms started to appear, and mRNA levels either kept constant or decreased after 72 h post-inoculation. To further investigate the putative involvement of the phenylpropanoid pathway in the defence of citrus fruit, changes in the metabolic profile of both tissues infected with P. digitatum was studied by means of HPLC-PDA-FD. Metabolite accumulation levels along the time course suggest that flavanones, flavones, polymethoxylated flavones and scoparone are induced in citrus fruit in response to P. digitatum infection, although with different trends depending on the tissue.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Ballester, Ana Rosa" sort="Ballester, Ana Rosa" uniqKey="Ballester A" first="Ana-Rosa" last="Ballester">Ana-Rosa Ballester</name></region><name sortKey="Gonzalez Candelas, Luis" sort="Gonzalez Candelas, Luis" uniqKey="Gonzalez Candelas L" first="Luis" last="Gonzalez-Candelas">Luis Gonzalez-Candelas</name><name sortKey="Lafuente, M Teresa" sort="Lafuente, M Teresa" uniqKey="Lafuente M" first="M. Teresa" last="Lafuente">M. Teresa Lafuente</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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